![]() Traditional bright-field imaging is suitable for observing commonly stained samples or strong absorption objects. ![]() As a statistical phenomenon of wave field, 1 the amplitude can be recorded by the digital detector (CCD or CMOS camera), whereas the phase, which carries important information about the object’s structure and optical properties, is lost. In microscopy imaging, the propagating wave field contains both amplitude and phase information by passing through some biological samples. Experimental results verify that a tunable lens-based TIE system, combined with the appropriate postprocessing algorithm, can achieve a variety of promising imaging modalities in parallel with the quantitative phase images for the dynamic study of cellular processes. Then we give the experimental demonstration of these ideas by time-lapse imaging of live HeLa cell mitosis. It makes the various observations for biomedical samples easy. We develop a requisite theory to describe such a hybrid computational multimodal imaging system, which yields quantitative phase, Zernike phase contrast, differential interference contrast, and light field moment imaging, simultaneously. Such information may provide tremendous flexibility to emulate various microscopy modalities computationally without requiring specialized hardware components. The quantitative phase reconstructed by TIE gives valuable information that has been encoded in the complex wave field by passage through a sample of interest. It does not require coherent illumination and works well on conventional bright-field microscopes. Transport of intensity equation (TIE) is a powerful tool for phase retrieval and quantitative phase imaging, which requires intensity measurements only at axially closely spaced planes without a separate reference beam.
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